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mouse monoclonal antibody against annexin a2 anxa2  (Proteintech)


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    Proteintech mouse monoclonal antibody against annexin a2 anxa2
    Mouse Monoclonal Antibody Against Annexin A2 Anxa2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 120 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+anxa2+monoclonal/Annexin+A2+Antibody/pm40943516-203-58-68
    Average 96 stars, based on 120 article reviews
    mouse monoclonal antibody against annexin a2 anxa2 - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Interaction of HE4 and ANXA2 exists in various malignant cells-HE4-ANXA2-MMP2 protein complex promotes cell migration.
    Article Snippet: .. After centrifugation at 15,000×g for 30 min at 4 °C, the supernatant was collected and treated with 10 μl of mouse anti-ANXA2 monoclonal (Proteintech, America, Cat. No. 66035-1-Ig) or goat anti-HE4 polyclonal antibody (Santa Cruz Biotechnology, Inc, America, Cat. No. sc-27570) for 3 h at 4 °C. .. Then, 20 μl of protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Inc) was added, followed by incubation on a rocker platform overnight at 4 °C.

    Article Title: Interaction of HE4 and ANXA2 exists in various malignant cells—HE4–ANXA2–MMP2 protein complex promotes cell migration
    Article Snippet: .. After centrifugation at 15,000× g for 30 min at 4 °C, the supernatant was collected and treated with 10 μl of mouse anti-ANXA2 monoclonal (Proteintech, America, Cat. No. 66035-1-Ig) or goat anti-HE4 polyclonal antibody (Santa Cruz Biotechnology, Inc, America, Cat. No. sc-27570) for 3 h at 4 °C. .. Then, 20 μl of protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Inc) was added, followed by incubation on a rocker platform overnight at 4 °C.

    Article Title: Expression and clinical significance of annexin A2 and human epididymis protein 4 in endometrial carcinoma
    Article Snippet: .. After centrifugation at 15,000 × g for 30 min at 4 °C, the supernatant was collected and treated with 2 μg of mouse anti-ANXA2 monoclonal (Proteintech, Chicago, America) or goat anti-HE4 polyclonal antibody (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) for 3 h at 4 °C. .. Then, 20 μl of protein A/G PLUS-Agarose (Santa Cruz Biotechnology, Inc.) was added, followed by incubation on a rocker platform overnight at 4 °C.



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    Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, <t>ANXA2</t> and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)
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    Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, <t>ANXA2</t> and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)
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    Image Search Results


    Journal: eLife

    Article Title: Proteomic landscape of tunneling nanotubes reveals CD9 and CD81 tetraspanins as key regulators

    doi: 10.7554/eLife.99172

    Figure Lengend Snippet:

    Article Snippet: Antibody , Mouse monoclonal anti-ANXA2 , Proteintech , #66035 , WB (1/2000).

    Techniques: Transfection, Construct, Expressing, Plasmid Preparation, Marker, Sequencing, Purification, Transduction, Control, Software, Staining

    Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, ANXA2 and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)

    Journal: BMC Genomics

    Article Title: Oviductal extracellular vesicles miRNA cargo varies in response to embryos and their quality

    doi: 10.1186/s12864-024-10429-5

    Figure Lengend Snippet: Characterization of extracellular vesicles (EVs). A Representative images of transmission electron microscopy (TEM) of EVs samples isolated from 6 experimental groups: EV_BGE: EVs from BOEC co-cultured with good-quality embryos; EV_BPE: EVs from BOEC co-cultured with poor-quality embryos; EV_B: EVs from BOEC cultured alone; EV_GE: EVs from good-quality embryos cultured alone; EV_PE: EVs from poor-quality embryos cultured alone; and M: medium only (control). Small images in each experimental group represent electron microphotographs of CD9 immunogold labelling of EVs. B Graphs representative for CD9 and CD63 expression in EVs samples, medium and PBS measured by flow cytometry. C Western blotting characterization of EVs and large vesicles (pellet after centrifugation of conditioned media at 12,000 g) for known EV protein markers (CD9, ANXA2 and HSP70). D Comparison of EVs size and concentration across samples measured by nanoparticle tracking analysis (NanoSight NS300)

    Article Snippet: Antibodies and their dilutions used for Western blotting experiments were as follows: primary antibodies: Anti-CD9 Mouse Monoclonal Antibody (Clone MM2/57, MCA469GT, Bio-Rad), 1:500; Anti-HSP70 Mouse Monoclonal Antibody (Santa Cruz sc-66048), 1:500; Anti-ANXA2 Mouse Monoclonal Antibody (Santa Cruz sc-28385), 1:500; secondary antibody: Anti-mouse m-IgGκ BP-HRP (Santa Cruz sc-516102), 1:10000.

    Techniques: Transmission Assay, Electron Microscopy, Isolation, Cell Culture, Control, Expressing, Flow Cytometry, Western Blot, Centrifugation, Comparison, Concentration Assay